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pan anti ip3r antibody  (Santa Cruz Biotechnology)
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Santa Cruz Biotechnology pan anti ip3r antibody
Fig.3. <t>IP3R</t> expression and knock down in HEK 293A cells. HEK 293A cells (2.5x105) were transfected with selective siRNAs against the different IP3R isoforms. 48 h after transfection, cells were lysed and their IP3R protein (panel A and B) or mRNA (panel C) levels were evaluated by Western blot and QPCR respectively. Panel A: Western blots showing the expression of the different IP3R isoforms (successively reprobing the same membrane) in cells transfected with siRNAs against IP3R-1 (1-1, 1-2, 1-3), IP3R-2 (2-1, 2-2, 2-3), IP3R-3 (3-1, 3- 2, 3-3) or transfected with a scrambled oligonucleotide (Sc). Panel B: Densitometric analysis of IP3R-1, IP3R-2, IP3R-3 and total IP3R bands shown in Panel A. Results are expressed as means ± SD of relative units of integrated peaks (ratio of IP3R/ HSP90 densities). Panel C: Relative levels of mRNAs encoding IP3Rs in cells transfected with selective siRNAs against the different IP3R isoforms (white bars) or transfected with a scrambled oligonucleotide (black bars). The results are expressed as % of total mRNAs (IP3R-1 + IP3R-2 + IP3R-3) measured in cells transfected with a scrambled oligonucleotide. These results are representative of at least three independent experiments. (* p<0.05 compared to control cells).
Pan Anti Ip3r Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Fig.3. IP3R expression and knock down in HEK 293A cells. HEK 293A cells (2.5x105) were transfected with selective siRNAs against the different IP3R isoforms. 48 h after transfection, cells were lysed and their IP3R protein (panel A and B) or mRNA (panel C) levels were evaluated by Western blot and QPCR respectively. Panel A: Western blots showing the expression of the different IP3R isoforms (successively reprobing the same membrane) in cells transfected with siRNAs against IP3R-1 (1-1, 1-2, 1-3), IP3R-2 (2-1, 2-2, 2-3), IP3R-3 (3-1, 3- 2, 3-3) or transfected with a scrambled oligonucleotide (Sc). Panel B: Densitometric analysis of IP3R-1, IP3R-2, IP3R-3 and total IP3R bands shown in Panel A. Results are expressed as means ± SD of relative units of integrated peaks (ratio of IP3R/ HSP90 densities). Panel C: Relative levels of mRNAs encoding IP3Rs in cells transfected with selective siRNAs against the different IP3R isoforms (white bars) or transfected with a scrambled oligonucleotide (black bars). The results are expressed as % of total mRNAs (IP3R-1 + IP3R-2 + IP3R-3) measured in cells transfected with a scrambled oligonucleotide. These results are representative of at least three independent experiments. (* p<0.05 compared to control cells).

Journal: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology

Article Title: The transcription factors NFAT and CREB have different susceptibilities to the reduced Ca2+ responses caused by the knock down of inositol trisphosphate receptor in HEK 293A cells.

doi: 10.1159/000322330

Figure Lengend Snippet: Fig.3. IP3R expression and knock down in HEK 293A cells. HEK 293A cells (2.5x105) were transfected with selective siRNAs against the different IP3R isoforms. 48 h after transfection, cells were lysed and their IP3R protein (panel A and B) or mRNA (panel C) levels were evaluated by Western blot and QPCR respectively. Panel A: Western blots showing the expression of the different IP3R isoforms (successively reprobing the same membrane) in cells transfected with siRNAs against IP3R-1 (1-1, 1-2, 1-3), IP3R-2 (2-1, 2-2, 2-3), IP3R-3 (3-1, 3- 2, 3-3) or transfected with a scrambled oligonucleotide (Sc). Panel B: Densitometric analysis of IP3R-1, IP3R-2, IP3R-3 and total IP3R bands shown in Panel A. Results are expressed as means ± SD of relative units of integrated peaks (ratio of IP3R/ HSP90 densities). Panel C: Relative levels of mRNAs encoding IP3Rs in cells transfected with selective siRNAs against the different IP3R isoforms (white bars) or transfected with a scrambled oligonucleotide (black bars). The results are expressed as % of total mRNAs (IP3R-1 + IP3R-2 + IP3R-3) measured in cells transfected with a scrambled oligonucleotide. These results are representative of at least three independent experiments. (* p<0.05 compared to control cells).

Article Snippet: Pan anti-IP3R antibody (recognizing an epitope common to all three IP3R isoforms) was from SantaCruz Biotechnology (Santa Cruz, CA ).

Techniques: Expressing, Knockdown, Transfection, Western Blot, Membrane, Control

Fig. 4. Reduced Ca2+ responses in IP3R- 2-knocked down HEK 293A cells. Attached siRNA- treated HEK 293A cells (2.5x105) were loaded with fura2/AM (0.1 µM), and stimulated with 3 µM CCh in the absence (panels A, B and C) or in the presence of 300 nM VIP (panels D, E and F). Panels A and D: Integrated Ca2+

Journal: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology

Article Title: The transcription factors NFAT and CREB have different susceptibilities to the reduced Ca2+ responses caused by the knock down of inositol trisphosphate receptor in HEK 293A cells.

doi: 10.1159/000322330

Figure Lengend Snippet: Fig. 4. Reduced Ca2+ responses in IP3R- 2-knocked down HEK 293A cells. Attached siRNA- treated HEK 293A cells (2.5x105) were loaded with fura2/AM (0.1 µM), and stimulated with 3 µM CCh in the absence (panels A, B and C) or in the presence of 300 nM VIP (panels D, E and F). Panels A and D: Integrated Ca2+

Article Snippet: Pan anti-IP3R antibody (recognizing an epitope common to all three IP3R isoforms) was from SantaCruz Biotechnology (Santa Cruz, CA ).

Techniques:

Fig. 5. VIP fully rescues CCh-induced maximal Ca2+ response in IP3R-2- knocked down cells. Attached siRNA treated HEK 293A cells (2.5x105) were loaded with fura2/AM (0.1 µM), bathed in HBSS and then stimulated with 1 mM CCh in the absence (panels A, B and C) or in the presence of 300 nM VIP (panels D, E and F). Panels A and D: Integrated Ca2+

Journal: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology

Article Title: The transcription factors NFAT and CREB have different susceptibilities to the reduced Ca2+ responses caused by the knock down of inositol trisphosphate receptor in HEK 293A cells.

doi: 10.1159/000322330

Figure Lengend Snippet: Fig. 5. VIP fully rescues CCh-induced maximal Ca2+ response in IP3R-2- knocked down cells. Attached siRNA treated HEK 293A cells (2.5x105) were loaded with fura2/AM (0.1 µM), bathed in HBSS and then stimulated with 1 mM CCh in the absence (panels A, B and C) or in the presence of 300 nM VIP (panels D, E and F). Panels A and D: Integrated Ca2+

Article Snippet: Pan anti-IP3R antibody (recognizing an epitope common to all three IP3R isoforms) was from SantaCruz Biotechnology (Santa Cruz, CA ).

Techniques: